Sample Set Annotation Tool: Identification of IL-21-induced STAT3 dependent genes in human B cells. GSE51587

Study Design
Assay
Sample Info
Files
Info
Samples
Group Sets
Analysis

Purpose

IL-21 induces B cell activation, and differentiation into antibody-secreting plasmablasts in vitro. This process is abolished by loss-of function mutations in STAT3We used microarrays to identify genes that are induced by IL-21 in a STAT3-dependent manner

Hypothesis

Experimental Design

Sort-purified naïve B cells from four healthy donors and three patients with AD-HIES due to heterozygous STAT3 mutations were cultured for four days with CD40L in the presence or absence of IL-21 (50ng/ml). After four days, RNA was extracted from the harvested cells and the genetic profile was analysed by microarray.

Experimental Variables

Controls

Methods

Additional Information

Expected Samples:

14 Samples Loaded: 14

Species

Human (Homo sapiens)

Sample Source

B cells

Disease

Stat3 deficiency

Treatment Protocol

Growth Protocol

Extraction Protocol

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	!Sample Title	!Sample_title	cell type	group	diagnosis
GSM1248745	na��ve B cells CD40L alone; donor 1	naïve B cells CD40L alone; donor 1	naïve B cells	Control B cells CD40L	healthy
GSM1248746	na��ve B cells CD40L alone; donor 2	naïve B cells CD40L alone; donor 2	naïve B cells	Control B cells CD40L	healthy
GSM1248747	na��ve B cells CD40L alone; donor 3	naïve B cells CD40L alone; donor 3	naïve B cells	Control B cells CD40L	healthy
GSM1248748	na��ve B cells CD40L alone; donor 4	naïve B cells CD40L alone; donor 4	naïve B cells	Control B cells CD40L	healthy
GSM1248749	na��ve B cells CD40L/IL-21; donor 1	naïve B cells CD40L/IL-21; donor 1	naïve B cells	Control B cells CD40L IL-21	healthy

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File
sampleset4000044_sampleannotations_Group.csv Sample Set Spreadsheet

Samples Viewer / Editor

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Sample ID	!Sample Title	!Sample Title	Cell type	Group	Diagnosis	Cell type
GSM1248745	na��ve B cells CD40L alone; donor 1	naïve B cells CD40L alone; donor 1	naïve B cells	Control B cells CD40L	healthy	na��ve B cells
GSM1248746	na��ve B cells CD40L alone; donor 2	naïve B cells CD40L alone; donor 2	naïve B cells	Control B cells CD40L	healthy	na��ve B cells
GSM1248747	na��ve B cells CD40L alone; donor 3	naïve B cells CD40L alone; donor 3	naïve B cells	Control B cells CD40L	healthy	na��ve B cells
GSM1248748	na��ve B cells CD40L alone; donor 4	naïve B cells CD40L alone; donor 4	naïve B cells	Control B cells CD40L	healthy	na��ve B cells
GSM1248749	na��ve B cells CD40L/IL-21; donor 1	naïve B cells CD40L/IL-21; donor 1	naïve B cells	Control B cells CD40L IL-21	healthy	na��ve B cells
GSM1248750	na��ve B cells CD40L/IL-21; donor 2	naïve B cells CD40L/IL-21; donor 2	naïve B cells	Control B cells CD40L IL-21	healthy	na��ve B cells
GSM1248751	na��ve B cells CD40L/IL-21; donor 3	naïve B cells CD40L/IL-21; donor 3	naïve B cells	Control B cells CD40L IL-21	healthy	na��ve B cells
GSM1248752	na��ve B cells CD40L/IL-21; donor 4	naïve B cells CD40L/IL-21; donor 4	naïve B cells	Control B cells CD40L IL-21	healthy	na��ve B cells
GSM1248753	na��ve B cells CD40L alone; AD-HIES pt 1	naïve B cells CD40L alone; AD-HIES pt 1	naïve B cells	AD Stat3 B cells CD40L	Autosomal dominant hyper IgE syndrome (AD-HIES)	na��ve B cells
GSM1248754	na��ve B cells CD40L alone; AD-HIES pt 2	naïve B cells CD40L alone; AD-HIES pt 2	naïve B cells	AD Stat3 B cells CD40L	Autosomal dominant hyper IgE syndrome (AD-HIES)	na��ve B cells
GSM1248755	na��ve B cells CD40L alone; AD-HIES pt 3	naïve B cells CD40L alone; AD-HIES pt 3	naïve B cells	AD Stat3 B cells CD40L	Autosomal dominant hyper IgE syndrome (AD-HIES)	na��ve B cells
GSM1248756	na��ve B cells CD40L/IL-21; AD-HIES pt 1	naïve B cells CD40L/IL-21; AD-HIES pt 1	naïve B cells	AD Stat3 B cells CD40L IL-21	Autosomal dominant hyper IgE syndrome (AD-HIES)	na��ve B cells
GSM1248757	na��ve B cells CD40L/IL-21; AD-HIES pt 2	naïve B cells CD40L/IL-21; AD-HIES pt 2	naïve B cells	AD Stat3 B cells CD40L IL-21	Autosomal dominant hyper IgE syndrome (AD-HIES)	na��ve B cells
GSM1248758	na��ve B cells CD40L/IL-21; AD-HIES pt 3	naïve B cells CD40L/IL-21; AD-HIES pt 3	naïve B cells	AD Stat3 B cells CD40L IL-21	Autosomal dominant hyper IgE syndrome (AD-HIES)	na��ve B cells

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