Dataset with samples from Individuals with:
A- Defects in Intrinsic and Innate Immunity:
1)- Herpes simplex encephalitis (HSE): UNC93B1 deficiency, Phenotype OMIM number 610551
2)- Predisposition to severe viral infection: IRF7 deficiency, Phenotype OMIM number Not yet assigned
see PID classification: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4659841/
- AR : Autosomal Recessive
IRF7 plays a critical role in the production and amplification of the antiviral type I and III interferon response. Autosomal recessive IRF7-deficiency resulted in life-threatening influenza disease in a 3-year-old child. We studied the impact of IRF7-deficiency in non-hematopoietic tissues (fibroblasts and lung epithelial cells) as well in hematopoietic cells (peripheral blood mononuclear cells (PBMCs)). Genome-wide gene expression analysis demonstrated a profound loss of type I and III IFNs in PBMCs infected with influenza virus.
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PBMCs were isolated from 4 healthy donors and patients with deficiencies for IRF7 and UNC93B. The cells were infected with influenza virus A/CA/4/2009 at a multiplicity of infection (MOI) of 2 for 8 and 16 hours. Uninfected cells were cultured for 16 hours.
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| Sample ID | !Sample Title | group | Cell type | Infected with | Subject status |
|---|---|---|---|---|---|
| GSM1623213 | Ctrl1 NS | Control No stimulus | PBMC | none for 16hrs (uninfected control) | healthy donor |
| GSM1623214 | Ctrl1 FLU_8h | Control Flu 8h | PBMC | influenza virus A/CA/4/2009 for 8hrs | healthy donor |
| GSM1623215 | Ctrl1 FLU_16h | Control Flu 16h | PBMC | influenza virus A/CA/4/2009 for 16hrs | healthy donor |
| GSM1623216 | Ctrl2 NS | Control No stimulus | PBMC | none for 16hrs (uninfected control) | healthy donor |
| GSM1623217 | Ctrl2 FLU_8h | Control Flu 8h | PBMC | influenza virus A/CA/4/2009 for 8hrs | healthy donor |
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| Sample ID | !Sample Title | Group | Cell type | Infected with | Subject status |
|---|---|---|---|---|---|
| GSM1623213 | Ctrl1 NS |
Control No stimulus |
PBMC |
none for 16hrs (uninfected control) |
healthy donor |
| GSM1623214 | Ctrl1 FLU_8h |
Control Flu 8h |
PBMC |
influenza virus A/CA/4/2009 for 8hrs |
healthy donor |
| GSM1623215 | Ctrl1 FLU_16h |
Control Flu 16h |
PBMC |
influenza virus A/CA/4/2009 for 16hrs |
healthy donor |
| GSM1623216 | Ctrl2 NS |
Control No stimulus |
PBMC |
none for 16hrs (uninfected control) |
healthy donor |
| GSM1623217 | Ctrl2 FLU_8h |
Control Flu 8h |
PBMC |
influenza virus A/CA/4/2009 for 8hrs |
healthy donor |
| GSM1623218 | Ctrl2 FLU_16h |
Control Flu 16h |
PBMC |
influenza virus A/CA/4/2009 for 16hrs |
healthy donor |
| GSM1623219 | Ctrl3 NS |
Control No stimulus |
PBMC |
none for 16hrs (uninfected control) |
healthy donor |
| GSM1623220 | Ctrl3 FLU_8h |
Control Flu 8h |
PBMC |
influenza virus A/CA/4/2009 for 8hrs |
healthy donor |
| GSM1623221 | Ctrl3 FLU_16h |
Control Flu 16h |
PBMC |
influenza virus A/CA/4/2009 for 16hrs |
healthy donor |
| GSM1623222 | Ctrl4 NS |
Control No stimulus |
PBMC |
none for 16hrs (uninfected control) |
healthy donor |
| GSM1623223 | Ctrl4 FLU_8h |
Control Flu 8h |
PBMC |
influenza virus A/CA/4/2009 for 8hrs |
healthy donor |
| GSM1623224 | Ctrl4 FLU_16h |
Control Flu 16h |
PBMC |
influenza virus A/CA/4/2009 for 16hrs |
healthy donor |
| GSM1623225 | UNC93B-/- NS |
AR UNC93B No stimulus |
PBMC |
none for 16hrs (uninfected control) |
patients with deficiencies for UNC93B |
| GSM1623226 | UNC93B-/- FLU_8h |
AR UNC93B Flu 8h |
PBMC |
influenza virus A/CA/4/2009 for 8hrs |
patients with deficiencies for UNC93B |
| GSM1623227 | UNC93B-/- FLU_16h |
AR UNC93B Flu 16h |
PBMC |
influenza virus A/CA/4/2009 for 16hrs |
patients with deficiencies for UNC93B |
| GSM1623228 | IRF7-/- NS |
AR IRF7 No stimulus |
PBMC |
none for 16hrs (uninfected control) |
patients with deficiencies for IRF7 |
| GSM1623229 | IRF7-/- FLU_8h |
AR IRF7 Flu 8h |
PBMC |
influenza virus A/CA/4/2009 for 8hrs |
patients with deficiencies for IRF7 |
| GSM1623230 | IRF7-/- FLU_16h |
AR IRF7 Flu 16h |
PBMC |
influenza virus A/CA/4/2009 for 16hrs |
patients with deficiencies for IRF7 |
| Name | ||
|---|---|---|
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